LV244A DATASHEET PDF
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(PDF) LV244 Datasheet download
All ORFs can be readily expressed in combination with a variety of fusion tags fluorescence, antibody, solubility, purification or no tagpromoters and selection markers, kv244a easy expression in different host systems.
Anti-tag antibodies available for easy detection. Uniformity datasheey high-quality clone sets make them ideal for large-scale high content and high-throughput screening studies in functional genomics, proteomics and systems biology. Check out more vector types of your choice if none of the above meets your needs. Please contact us at or inquiry genecopoeia. Add this page to bookmarks View Bookmarks Delete this bookmarks.
Popular tags and technologies. Overpremade ORF clones. Premade clones grouped into gene families or custom groups. All ORF clones are sequence validated. See our clones in publications! These clones are compatible with both recombination and traditional cloning using multiple cloning sites MCS. Control clones — Include positive and negative control clones that can be purchased together with or separately from your clone of interest. All you need to do is go to thesearch for your gene, and then choose the appropriate clones that will work for your system.
If you have any custom requirements, then you will need to and, after determining what you need, we will send you a custom quote. What delivery formats do you offer for your ORF clones?
You can also request alternative delivery formats by contacting us at support genecopoeia.
It is available at a competitive and ships next day if the order is placed by noon Eastern Time. What is the turnaround time for your ORF clones? The turnaround time for ORF clones varies greatly, depending on many factors. Most other clones have a turnaround time of about weeks. If we need to make any customizations, or if datasueet encounter any unforseen technical difficulties, the turnaround time could be longer.
What is the “Secondary Clone Discount”? Once the clone is constructed, GeneCopoeia follows a rigorous quality control process to ensure the correct clone is delivered to you. We first amplify the sequences by PCR and validate the size. Next, all ORFs are fully sequenced.
Restriction enzyme digestion is used to check the integrity of the entire plasmid. The DNA insert datasheft only the protein coding sequence from start codon to stop codon of a full-length gene or cDNA without 5′ and 3′ end untranslated regions UTRsor introns. GeneCopoeia guarantees that our OmicsLink TM ORF Expression clone sequences are free of artificial frame-shifting variations, including deletionss and additionss of bases, as well as translation termination mutations non-stop codon to stop codon resulting from point mutation.
If you want a specific version of a gene from different cDNA libraries, GeneCopoeia will make its best effort to obtain that particular version but without guarantee of success. Dataxheet expression system should be specified first. We recommend the lentiviral system if the mammalian target cell is hard to transfect. Next, tags should be chosen.
GeneCopoeia provides fluorescent tags, solubility and purification tags, antibody immunoprecipitation tags and multifunctional tags.
How do I decide between N-terminal tags and C-terminal tags? Tags should be placed far from the active center and functional domains of the target protein. If the target protein has an N-terminal signal peptide, then the tag must be placed at the C terminus.
These new vectors can then be used to express the desired protein in the targeted cell type. What restriction sites are available for subcloning into other vectors?
This information can be found in the clone datasheet you received. Currently, we offer pre-made human, mouse, and zebrafish ORF clones. We are in the process of producing full length ORF clones from other model animals such as rat.
These full-length ORF clones will be released to market in the near future. If you are interested in a full length ORF of any genes of interest from other model organisms, please send us the information of these genes, such as GenBank Accession or Nucleotide sequences. They will be charged with the same price as our other catalog ORF clones.
We also offer custom cloning services for cloning genes from other model organisms into any vector of your choice. I have done an expression experiment, but there is no protein.
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A correct DNA sequence with a promoter does not guarantee you will get protein expressed. Many factors contribute to the success eatasheet failure of protein expression: For instance, our pReceiver-M01 vector is for mammalian lv244z expression and pReceiver-B01 is for bacterial expression.
Have you dtasheet expression tests of each gene? Other expression clones with different features and for different host cell systems have not been tested for protein expression. After I have done full-length sequencing of your clone, I found it is an alternative splicing form of the gene I am interested in, can you reclone and replace it? We will make the best effort to clone the original splicing form and provide you with the replacement clone. However, we cannot guarantee that we will get the clone of a specific splicing variant.
LVA Datasheet, PDF – Alldatasheet
There is one nucleotide change in the sequence, and it encodes an amino acid for proper protein function as cited by many publications. How will you handle this case?
Even though we cannot provide replacement clones for this type of case, we do offer mutagenesis services to change any nucleotide composition as requested for a nominal fee.
We guarantee that there are no frame-shifting changes and no premature stop codons in our full length ORF inserts dtaasheet with reference sequences. We will replace defective clones or refund the charges if that occurs. How do you explain the differences between your actual sequencing data and GenBank sequences?
The differences daatasheet originate from two potential sources or a combination of the two: Very often, potential users of our clones compare our sequencing data with the sequence of only one reference transcript, and attribute any discrepancies to PCR datadheet mutations. Typically, we found that there are datashet discrepancies between the sequences of our ORF clones and the corresponding genomic sequence than the sequences of public domain gene transcripts and the corresponding genomic sequences.
We certainly cannot entirely eliminate the possibility of mutations introduced by our PCR cloning process, but we believe they are rare occurrences. We offer services at a small fee for changing base nucleotide compositions. How do you generate full length ORFs to produce your clones?
I would like to sequence the ORFs. What primer sequences can I use? To download the datasheet, please visit the section on our website. Please note that you will lv24a4 the following information in order to download your data sheets: Login information for your account on our website, the catalog number datasheey of your clone sand your sales order number SO.
If you are unable to download the datasheets from our website, please contact Technical support. How do you do quality control of your clones? Each full length ORF insert is fully sequenced. Before the delivery of each clone, we re-check the clone by PCR with gene-specific primers to ensure that the correct clone is shipped. I cannot find my genes of interest from searching the database in your website. Can you clone lg244a gene for me? How much will it cost?
Yes, we will clone the gene for you. It is our mission and continuing effort to clone all full-length ORFs of human lv244s. It is likely that the gene that you are interested in is already in our production pipeline or in the queue for future cloning.
If you provide us with the GenBank accession number, gene name, nucleotide or amino acid sequences, we will place the gene in the list of priority genes to be cloned. Usually, it takes two weeks to complete the cloning, although we cannot guarantee that we will get the gene.
You will be notified as soon as the lv24a becomes available. There will be no additional charge. In some cases, when comparing your reference sequences with those from NCBI with the same gene symbol or description, Datashdet notice there datasheett differing degrees of discrepancies between the sequences. Can you explain the discrepancies? There are usually multiple gene transcripts in a gene locus, resulting from alternative splicing, updated information about ORFs, polymorphisms, or redundancies from duplication of cloning efforts from different laboratory groups.
During the process of selecting full-length genes for ORF cloning, we try to choose the most representative transcript from each gene locus, which may differ slightly or significantly from other transcripts in the same gene locus. This datasheft provides the broadest coverage of all gene loci. In many instances, we do obtain multiple variants or versions of full length ORFs from our cloning production pipeline.
Customers are encouraged to send inquires to inquiry genecopoeia. No, the sequence on the website is not the actual sequence but the reference sequence from NCBI.
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The actual sequence of the clone may differ due to genetic variation and alternative splicing. However, please note that the actual sequence for clones in pre-production or clones offered via Custom Gene Synthesis may not yet be available. First, check the datasheet that is shipped with the clone and use the figure to identify the sequence. Multiple cloning site of Datssheet No. There are various options for the selections.