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ENDOSAFE PTS PDF

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The Endosafe®-PTS™ is a handheld spectrophotometer that utilizes FDA- licensed disposable cartridges for accurate, convenient endotoxin testing. With test. A multicenter comparison study between the Endosafe PTS rapid-release testing system and traditional methods for detecting endotoxin in cell-therapy products. The Endosafe®-PTS™ is a rapid, point-of-use test system that provides quantitative LAL results within 15 minutes. The PTS™ utilizes LAL reagents in an .

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The goal of the experiment is to design and test procedures to monitor and document the spread of biological material associated with human spaceflight, both before and after launch, in preparation for the human exploration of the Moon and Mars.

From Wikipedia, the free encyclopedia. Nedosafe Read Edit View history. Retrieved from ” https: Endotoxin and glucan are detected with the Limulus amebocyte lysate LAL enzyme cascade, derived from the horseshoe crab Limulus polyphemus. Rapid-release testing reduces the waiting period for administration of time-sensitive cell-therapy products.

Endosafe-PTS Glucan Assay from Charles River – Product Description and Details

Gravitational Physiology, Volume 10, Issue 2, Traditional culture-based methods aboard the ISS take several days and often require return of samples to Earth for more in-depth analysis by scientists on the ground.

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The projects develop integrated lab-on-a-chip products in three areas related to space exploration: Current assay systems are labor intensive and time consuming. The Endosafe portable test system PTS is a chromogenic Limulus amebocyte lysate LAL portable endotoxin detection system that provides quantitative results in approximately 15 min. Archived copy as title Commons category link is on Wikidata. Before LOCAD-PTS was launched to space, it was tested extensively in simulated microgravity with the use of parabolic flight endosare, to verify nominal function of the instrument and sample handling procedures.

In other projects Wikimedia Commons. This ‘swab’ was performed indirectly with the spacesuit gloves.

Endosafe nexgen-PTS from Charles River – Product Description and Details

An endosaafe can take a swab sample from a cabin surface, dissolve it in endotoxin-free water, and analyze it quantitatively with LOCAD-PTS in microgravity, all in under 15 minutes. Endotoxin detection between paired results was equivalent in most cases.

The Endosafe system provided reliable results with products typically produced in cell-therapy manufacturing facilities, and would be an appropriate test on which to base the release of time-sensitive cell-therapy products.

Valid paired results, based on each center’s LAL method and the Endosafe system criteria, were analyzed. Karissa West performs microbial survey of Pad 39A August 7, Archived from the original on All samples tested with the Endosafe system used 0.

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By the amplification of a small signal, these enzyme cascades allow detection of very low amounts of starting microbial material. Endotoxin was found at every surface site where Colony Enxosafe Units CFUs were observed with culture-based contact slide methods.

Endosafe-PTS Glucan Assay from Charles River

Preliminary results with these cartridges showed similar patterns to those observed for endotoxin, but glucan was found at higher levels and found to be endowafe more widespread throughout the ISS. This page was last edited on 8 Novemberat By using this site, you agree to the Terms of Use and Privacy Policy.

Additionally, fabricated endotoxin-positive samples containing varying concentrations of endotoxin were prepared and shipped to all centers to perform blinded testing. Subsequently, each center tested the endotoxin content of standard dilutions of cell-therapy products, thus creating paired test results for each sample. The device itself or reader weighs 2.

These three molecules are found mainly within gram-negative bacteriafungi and gram-positive bacteriarespectively.

Tests with these cartridges have been performed during Expeditions 18 —